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Cytotoxicity assays Cells were plated into a 96-well plate at a density of 110 4 cells per well and incubated in a cell incubator (37 C, 5% CO 2 )
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Targeting this system, either by hepatocyte-specific knockout of peroxisomes or by deficiency in ACOX (which catalyzes the initial step in peroxisomal FAO), results in hepatic lipid accumulation and fibrosis, oxidative stress, and inflammation, emphasizing the role of peroximal FAO in NAFLD and NASH [108]
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