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If stubborn chunks remain after 5 minutes, let the vial sit upright in the fridge for 15 to 30 minutes, then roll again

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Impact of ATXN3 Misfolding on the Subcellular Compartments: Mitochondria and ER Wild-type and mutant ATXN3 proteins have also been identified in the mitochondrial protein fraction of cells [138, 139], suggesting that mitochondrial function may be impaired as a result of ATXN3 misfolding and aggregation

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