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After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody

Specifications: Unit Size: 10mg (lyophilized powder, single vial) Molecular Formula: CHNO Molecular Weight: ~1419.5 Da Form: Lyophilized powder Storage: Store lyophilized peptide at 2C8C in a cool, dark place

Authors concluded that, in the process of prostate carcinogenesis, activating histone modifications on GSTP1 are lost and subsequently DNA becomes methylated and inaccessible resulting in transcriptional silencing thus demonstrating interplay between histone reprogramming and promoter region methylation (Okino et al., 2007)

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