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Enzyme-Linked Immunosorbent Assay (ELISA) Active TGF-1 expression was measured using an ELISA kit (Boster, Wuhan, China), according to the manufacturers instructions

In laboratory environments, researchers may review its peptide sequence, analytical identity, purity profile and stability characteristics

The uterine and kidney tissues were removed from experimental rats and subjected to western blot analysis

In conclusion, intestinal fungal dysregulation, mycobiome-derived metabolites, and the hosts antifungal immune response are key factors in the severity and prognosis of ALD, providing promising targets for future antifungal or immunomodulatory treatments