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leu)7697 galU galK λ- rpsL (StrR) nupG
The device is fully specified for sample transport and storage
0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA)
we recommend the MasterAmp™ PCR Optimization Kit with ammonium sulfate for rapid PCR optimization
Exonuclease VII, 250 U @ 10 U/uL ISOBUC leu)7697 galU galK λ- rpsLDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease
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