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Ferroptosis is characterized by dysregulated iron metabolism, lipid peroxidation, and reactive oxygen species (ROS) accumulation, processes intimately linked to CHD pathophysiology

Proteinase K was added simultaneously to all samples at a 1:100 enzyme/substrate ratio and incubated at 25 C for 5 min

Abbreviations APC: Adenomatous polyposis coli CDK5RAP2: Cyclin-dependent kinase 5-related activator protein 2 EB1: End-binding protein-1 ERK: Extracellular signal-regulated kinase CLASP: Cytoplasmic linker protein (CLIP)-associated protein FAK: Focal adhesion kinase GCP: -tubulin complex protein GSK-3: Glucogen synthase kinase-3 ILK: Integrin-linked kinase IQGAP: IQ motif-containing GTPase-activating protein KANK: KN motif and ankyrin-repeat-containing protein KIF21A: Kinesin family member 21A LL5: Pleckstrin homology-like domain family B member 2 mDIA: Diaphanous-related formin-1 MMP: Matrix metalloproteinase MOZART: Mitotic spindle organizing protein associated with a ring of -tubulin NEDD1: Neural precursor cell expressed, developmentally down-regulated-protein 1 PAK: p21-activated kinase PAR6: Partitioning-defective-6 PKC: Protein kinase C RSK2: Ribosomal-S6 kinase-2 References Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines

The 28-day reconstituted shelf life assumes zero freeze-thaw cycles post-reconstitution