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Because translation is more efficient, the set of available chaperones to assist in protein folding becomes saturated and this may cause the accumulation and aggregation of misfolded proteins in the cytoplasm (28)

The difference between adding 1mL and 2mL of bacteriostatic water does not change the total amount of peptide in the vial, but it dramatically changes how precisely you can measure each dose and how many weeks that vial will last in your refrigerator