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Additionally, chronic conditions such as HIV and tuberculosis have been implicated in B12 deficiency due to increased metabolic demand and altered absorption mechanisms (Green, 2017)

The supernatant was discarded and the beads were washed thrice with LSB-2 containing 150 mM NaCl which is termed LSB-3 (10 mM Tris pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 150 mM NaCl, and 1 mM PMSF) with or without IGEPAL CA-630 followed by incubation at 4 C/1 h