It is to build a strategy around the individual, the objective, the clinical data, and whether the approach is actually working
This step equalizes pressure and prevents vacuum formation, which would otherwise make it difficult to draw liquid and would pull contaminants back through the needle on subsequent draws
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Finally, the absence of validated biomarkers for predicting treatment response or monitoring efficacy further complicates both clinical research and practical application, limiting the ability to personalize therapy or objectively assess outcomes