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Five hundred cells of KISPIDMG-105, 500 cells of SF8628, or BBB cells were loaded into each well of an Akura 384 or Akura 96 spheroid microplate (InSphero AG, Schlieren, Switzerland) and centrifuged at 250 g for 3 min

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The authors emphasize, however, that the fact that TL only modestly contributed to the composite biomarker of aging can lead to misleading interpretation of the study results, depending on whether they are used for the prediction or explanation of biological aging