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The cells were then collected by centrifugation, washed with cold PBS and incubated with annexinV-Pacific Blue in annexin-binding buffer at 20 C for 15 min

This is another reason to use reconstituted peptides within the 28-day window, not just for sterility but for dosing consistency

Thus, our results confirm earlier conclusions that CP-bound copper ions are practically nonexchangeable at physiological pH values and CP does not participate in the regulation of exchangeable copper levels in the blood 5

Melatonin in assisted reproductive technology: a pilot double-blind randomized placebo-controlled clinical trial