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For generation of the GRASP55 7 S* mutant, identified serines and adjacent serines S3, S4, S449, S451 were mutated to threonine to minimally effect the physio-chemical properties of these amino acids, in addition, S408, S409, S441 were mutated to alanine by site-directed mutagenesis

Results Fragment-based drug design and chemistry Utilizing actives identified in a quantitative high-throughput screen against Schistosoma mansoni TGR 20,35 , 92 commercially available low molecular weight (MW) compounds/fragments were tested by X-ray crystallography 33,34,36

And the folate, vitamin B6, and vitamin B12 involved in converting homocysteine into methionine