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Use a 0.3 mL insulin syringe (30 units) with fine gradations for the most precise measurements at this concentration

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Using a camera attached to a USB microscope (Veho discovery VMS-004 deluxe), we recorded the vessel presentation (fulfilled/appearance or cleared out/disappearance) between the arcade vessels on the ventral and dorsal sides throughout the following 15 min [with regard to the point immediately before therapy (as 100%)] at selected time points before and after therapy [see the assessment shown in Figure 1 (IC rats)]