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Kong, L., Wu, Q

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Alveolar epithelial cells appear to be remarkably tolerant to oxygen deprivation as they are able to maintain adequate cellular ATP content during prolonged hypoxic exposure [12] and hence considered as an elegant gatekeeper between environmental hypoxia and physical performance at high altitude [8,13]

Subsequently, the cells were co-incubated overnight with an anti-cytochrome c antibody, followed by a 2-h incubation with an Alexa Fluor 488 conjugated secondary antibody