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Kozai T, Kubota CJB (1997) Environmental control for the large-scale production of plants through in vitro techniques

For promoter analysis, 2 kb upstream region from translation start code ATG of each GhGPXs was from database of CGP 39 and another database 41 , and then predicted cis -acting elements in (+) strand and () strand of each promoter of GhGPXs was found using the database of PlantCARE 42 (Plant Cis -Acting Regualtory Element, Gene expression analysis Total RNA was extracted by using TRIzol reagent (Invitrogen) from G

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Do not double the next dose sustained protocols rely on cumulative tissue accumulation, and catching up with excess dosing provides no accelerated benefit while increasing side effect probability