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Lipid bilayer was placed at centre in a rectangular periodic box filled with TIP3P water and ZIL (with 8716 and 32 molecules, respectively), where the buffer size between the edges of the box and the lipid model was set at around 34 , and the MD simulation of cell membrane in 5 wt% ZIL aqueous solution was carried out using Amber18 and AmberTools19 software 31

To minimize variability, samples were analysed in a single batch and randomized to mitigate potential LC-MS system drift effects over the duration of the analysis

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A narrow and uniform pore size distribution is indicated by the overlapping adsorption/desorption curves [28]