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For simulated ischemia/reperfusion (sI/R), ischemia was imposed by a buffer exchange to ischemic solution (20 mM HEPES, pH 6.6, 20 mM deoxyglucose, 125 mM NaCl, 8 mM KCl, 1.2 mM KH 2 PO 4 , 1.25 mM MgSO 4 , 1.2 mM CaCl 2 , 6.25 mM NaHCO 3 , 5 mM sodium lactate) and placing in a humidified chamber equilibrated with 95% N2 and 5% CO2

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