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The amount of cartridge use in milliliters per month was represented by medians and interquartile ranges (IQR) 25 to 75 as it did not follow a normal distribution

Cells were pre-washed in 0.1 M cacodylate buffer and post-fixed in 1% OsO4 supplemented with 1.5% K3Fe(Cn6) for 2 hours, dehydrated and embedded in epoxy resin according to routine procedures