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Without proper anatomical landmarks and sterile technique, you risk introducing bacteria into a synovial space where infection (septic arthritis) can destroy cartilage within 24-48 hours

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[1] Cell Line:Cultured Sprague-Dawley rat Achilles tendon fibroblasts (passages 2-4) Concentration:2 g/mL Incubation Time:24 h Result:Increased F-actin (polymerized actin fibers, visualized as green fluorescence) levels notably relative to control cells with weak F-actin detection