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Incubate the plate at 30C for 10 min

doi: 10.3945/ajcn.113.065789 129 PoutonCWPorterCJ

(Its not clear if the peptides were responsible for the symptoms.) Considering the skin-darkening peptide melanotan II (sometimes called the Barbie peptide)

We found that phosphorylation of the S114 priming residue altered the nucleocytoplasmic distribution of NHR-49 and was necessary for subsequent phosphorylation of adjacent residues by recombinant KIN-19 (Figs