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Subsequent cell culture studies revealed that GSSG accumulation, as opposed to GSH reduction, sensitized hepatocytes to TNF killing by reducing the TNF-triggered NF-B activity

Das S, Ciombor KK, and Haraldsdottir S, et al (2020) Immune-related adverse events and immune checkpoint inhibitor efficacy in patients with gastrointestinal cancer with Food and Drug Administration-approved indications for immunotherapy Oncologist 25 (8) 669679 PMID: 31943525 PMCID: 7418359 62

Therefore, Erastin not only directly induces necroptosis but also amplifies its occurrence through the ROS generated during ferroptosis, establishing a synergistic interaction between ferroptosis and necroptosis

DTNB (5,5-Dithiobis(2-nitrobenzoic acid)) and glutathione (GSH) react to generate 5-mercapto-2-nitrobenzoic acid and glutathione disulfide (GSSG)