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Activation of the cAMP/PKA pathway induces loss of stress fibers (112), activation of regular arrangement of collecting venules (Rac) and Cell Division Cycle 42 (Cdc42) (113, 114), formation of filamentous pseudopods and lamellar pseudopods (114, 115) microfilament assembly (116), and inhibition of the Rho family of GTPases activity (117, 118), events which happen at the tip of migrating cells

The number of viable cells was measured by flow cytometry after addition of 5 l spike in fluorescently labeled bead (CountBright Plus Absolute Counting Beads, ThermoFisher Scientific) for normalization