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Lung tissue was processed as follows: one portion was immediately frozen in liquid nitrogen and stored at 80 C for gene expression analysis
Then, cells were incubated with a 1:200 dilutions of primary anti--H2AX antibody (ABclonal Technology, Wuhan, China) overnight at 4 C, followed by three washes with PBS and incubation with FITC-labeled goat anti-rabbit IgG antibody for 1 h and mounted with DAPI [32]
Despite their potential, polyphenols remain underutilized in functional foods and dietary supplements due to several physicochemical properties, including low epithelial permeability, poor solubility in gastrointestinal fluids, structural modifications during digestion, and limited oral bio-accessibility (128, 129)
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