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Two earlier sub-chronic nicotine studies found no alterations in GLT-1 or xCT in the prefrontal cortex of rats given nicotine orally or through minipumps for 21 days (Redcay and Courchesne, 2008), or in mice exposed intermittently to nicotine-containing e-cigarette vapor ( via cigarette smoke downregulated GLT-1 in the NAc and xCT expression in the PFC and the NAc