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Because no specific clinical or laboratory features of HRS have been identified, diagnosis depends on (1) the presence of severe liver disease, (2) a rise in creatinine to greater than 1.5 mg/dL (25.6 mol/L), (3) no evidence of other renal disease by urinalysis and clinical history, and (4) lack of improvement in renal function with treatments that increase intravascular volume (such as stopping diuretics, or administration of fluids and/or albumin)

The day before the experiment, two million cells were seeded in 10 cm dishes

[82] Lateral hypothalamic orexin neurons innervate every component of the ascending reticular activating system (ARAS), which includes noradrenergic, dopaminergic, histaminergic, and serotonergic nuclei that promote wakefulness

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