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Purification by reverse-phase chromatography brings the peptide to the 99% release specification, after which lyophilization into 10 mg vials completes the build cycle on Canadian soil

Reverse transcription was performed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific) with 1,000 ng of RNA following the manufacturer's instructions and using a ProFlex PCR System (Applied Biosystems, Thermo Fisher Scientific) machine

B12 Absorbed in the Small Intestine: In the duodenum, B12 separates from haptocorrin and binds to another protein called intrinsic factor (IF)

This neurotrophic system is described in the next section ( Figure 1 )