The following experimental conditions were applied: CE mode: CZE Electrolyte: Phosphate, pH 1.8 (adjusted with phosphoric acid (85 %) to fulfill the SST) Capillary: fused silica, 75 m ID, 64.5 cm total length Temperature: 25C Injection: 5 s, 50 mbar Detection: direct UV, 200 nm Separation: 20 kV Description: The method is used for testing the purity of glutathione by determining the related substances L-cysteinylglycine, L-cysteine, L-glutathione oxidized, L-y-glutamyl-L-cysteine and unspecified impurities
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By disrupting systemic oxidative balance and compromising cellular integrity, ferroptosis adversely influences follicular development, ovulation, and hormonal regulation, thereby playing a critical role in the pathophysiology of PCOS