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(AGAGTTTGATCCTGGCTCAG) to 1492r (CACGGATCCTACGGGTACCTTGTTACGACTT) region of the 16S rRNA gene was amplified in triplicate using 100200 ng of DNA template with 2U of Taq, 1 buffer, 1.5 mM MgCl 2 , 0.4 mg/mL BSA, 0.2 mM dNTPs, 50 g/mL RNaseA, and 10pmols of each primer

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J Gen Intern Med 2018;33(7):11311138