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Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to aluminum stress in transgenic tobacco

Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

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Treatment of mouse heart homogenates generated under native conditions with Na 2 S 2 also led to the formation of PrSS and PrS(S) n S detected by the same procedures (Supplementary Fig