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Cortices were then dissociated using the Primary Neuron Isolation Kit and triturated in growth media with serum and then transferred to a solution of warm Opti- | Reduced Serum Medium, with glucose (Gibco/Fisher Scientific, Cat# 31-985-062) for centrifugation

Smaller or more delicate peptides may require use within two weeks

Animal models show anti-anxiety-like and pain-reducing effects, probably running through GHK-Cus broad gene modulation and inflammation pathways rather than any direct hit on neuroreceptors

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