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DF, JB, and AS contributed the reagents, materials, and analysis tools

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148 This has led to the development of the web-based metalation calculator tool, which allows a user to input known metal binding equilibrium constants for their protein of interest and calculates the degree to which that protein will metalated with a particular metal in an idealized cytoplasm. 146,152 The lack of an unbiased validation of the predictions of this model, however, remains both a significant limitation and a considerable analytical challenge, for two reasons

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